Serological, genomic and structural analyses of the major mite allergen Der p 23

Mueller GA, Randall TA, Glesner J, Pedersen LC, Perera L, Edwards LL, DeRose EF, Chapman MD, London RE, Pomés A

Clin Exp Allergy. 2016 Feb;46(2):365-76. 

Abstract

BACKGROUND:

Der p 23 was recently identified in a European population as a major allergen and potentially a chitin binding protein.

OBJECTIVE:

This study sought to assess the importance of Der p 23 among other Dermatophagoides allergens in a North American population and to determine the structure for functional characterization.

METHODS:

IgE binding to Der p 23, Der p 1, Der p 2, Der p 5, Der p 7 and Der p 8 was measured by ELISA. RNA-seq data from D. pteronyssinus were compared as estimates of allergen expression levels. The structure was analysed by X-ray crystallography and NMR.

RESULTS:

Despite a high prevalence of Der p 23, (75% vs. 87% and 79% for Der p 1 and Der p 2, respectively), the anti-Der p 23 IgE levels were relatively low. The patient response to the 6 allergens tested was variable (n = 47), but on average anti-Der p 1 and anti-Der p 2 together accounted for 85% of the specific IgE. In terms of abundance, the RNA expression level of Der p 23 is the lowest of the major allergens, thirty fold less than Der p 1 and sevenfold less than Der p 2. The structure of Der p 23 is a small, globular protein stabilized by two disulphide bonds, which is structurally related to allergens such as Blo t 12 that contain carbohydrate binding domains that bind chitin. Functional assays failed to confirm chitin binding by Der p 23.

CONCLUSIONS AND CLINICAL RELEVANCE:

Der p 23 accounts for a small percentage of the IgE response to mite allergens, which is dominated by Der p 1 and Der p 2. The prevalence and amount of specific IgE to Der p 23 and Der p 2 are disproportionately high compared to the expression of other Dermatophagoides allergens.

KEYWORDS:

Der p 23; RNA-seq; allergen; house dust mite

Abstract on Pubmed

Published 2015. This article is a U.S. Government work and is in the public domain in the USA.

KEYWORDS:

Der p 23; RNA-seq; allergen; house dust mite